Indirect immunofluorescent assay as an aid in the diagnosis of suspected immune mediated ataxias
نویسندگان
چکیده
Abstract Background and purpose Immune mediated cerebellar ataxias account for a substantial proportion of all progressive ataxias. A diagnostic serological test is not always available. This particularly problematic in Primary Autoimmune Cerebellar Ataxia, hence the necessity criteria recently devised published by an International Task Force. We present our experience use commercially available indirect immunofluorescence assay, intended to be used detection antibodies associated with paraneoplastic neurological syndromes. Methods Retrospective review patients ataxia who underwent testing using this assay as part their evaluation. were interested 3 groups: suspected immune ataxias, genetically confirmed variant multi-system atrophy (MSA-C). The was performed monkey cerebellum slides anti-human IgG FITC conjugated antiserum. Results total 300 that had fitted into one these groups (immune 190, genetic 60, MSA-C 50) identified. prevalence positive but negative immunoblot 172/190 (91%) group, 3/60 (5%) group 2/50 (4%) group. difference between first other significant χ 2 (1, N = 291) 64.2, p < 00001. Conclusions report demonstrates can provide additional aid particular Ataxia where no marker exists.
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ژورنال
عنوان ژورنال: Cerebellum & ataxias
سال: 2021
ISSN: ['2053-8871']
DOI: https://doi.org/10.1186/s40673-021-00129-1